Coordinated Regulation of Gene Expression by E.coli Chromatin Proteins FIS and H-NS

Released on by Ramesh Mavathur Nanjundaiah
preview-18

  • Coordinated Regulation of Gene Expression by E.coli Chromatin Proteins FIS and H-NS Book Detail

  • Author : Ramesh Mavathur Nanjundaiah
  • Release Date : 2007
  • Publisher :
  • Genre :
  • Pages : 176
  • ISBN 13 :
  • File Size : 8,8 MB
DOWNLOAD BOOK

Coordinated Regulation of Gene Expression by E.coli Chromatin Proteins FIS and H-NS by Ramesh Mavathur Nanjundaiah PDF Summary

Book Description: Bacteria posses impressive ability to adapt to their environment. Adaptation is accomplished mainly by adjustment of the gene expression pattern. Studies on transcript profiling in E.coli demonstrated the existence of direct correlations between the global gene expression patterns and different phases. Previous work indicated that in E.coli the transcription is controlled by a global regulatory network whose main components are - DNA topoisomerases, chromatin proteins and the RNA polymerase. Aim of this project was to understand the structural mechanism of interaction of the components (DNA supercoiling and chromatin proteins) and the underlying functional coordination of gene expression in E.coli. To understand the molecular mechanism of the interplay, the problem was approached at two levels of complexity (1) global - using a novel DNA microarray-based approach combining mutations in the genes of chromatin proteins with directional changes of DNA superhelicity and (2) local - at the level of individual gene promoter, using tyrT promoter system. The study has led us to propose that the global transcription is spatiotemporally coordinated by the differential distribution of two types of information, 'analog' and 'digital'. The digital or discontinuous property of information is manifest in the pattern of expressed genes depending on the distribution of the analog component - DNA supercoiling - in different parts of the genome. The analog component is continuously converted in the pattern of expressed genes and vice versa, thereby modulating the physiology to a dynamic equilibrium. On the example of tyrT promoter we demonstrate how the sequence organization of the promoter determines the supercoiling sensitivity of the promoters. We also show that the transcription from a strong promoter, like tyrT, is a consequence of the local effect of binding of chromatin protein FIS at the promoter and the general effect of FIS on the average superhelical density of the DNA.

Disclaimer: www.yourbookbest.com does not own Coordinated Regulation of Gene Expression by E.coli Chromatin Proteins FIS and H-NS books pdf, neither created or scanned. We just provide the link that is already available on the internet, public domain and in Google Drive. If any way it violates the law or has any issues, then kindly mail us via contact us page to request the removal of the link.

Bacterial Physiology

Bacterial Physiology

File Size : 81,81 MB
Total View : 6169 Views
DOWNLOAD

The application of new molecular methodologies in the study of bacterial behavior and cell architecture has enabled new revolutionary insights and discoveries i

The Bacterial Chromosome

The Bacterial Chromosome

File Size : 51,51 MB
Total View : 3394 Views
DOWNLOAD

An examination of the mechanisms governing genetic inheritance. - Provides a link between classical experiments in chromosome physiology and new developments in

Manual on MUTATION BREEDING THIRD EDITION

Manual on MUTATION BREEDING THIRD EDITION

File Size : 17,17 MB
Total View : 3149 Views
DOWNLOAD

This paper provides guidelines for new high-throughput screening methods – both phenotypic and genotypic – to enable the detection of rare mutant traits, an