DNA Cloning: A Hands-on Approach

Released on by Seok-Yong Choi
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  • DNA Cloning: A Hands-on Approach Book Detail

  • Author : Seok-Yong Choi
  • Release Date : 2019-04-17
  • Publisher : Springer
  • Genre : Medical
  • Pages : 131
  • ISBN 13 : 9402416625
  • File Size : 72,72 MB
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DNA Cloning: A Hands-on Approach by Seok-Yong Choi PDF Summary

Book Description: This book offers step-by-step instruction on DNA cloning, defined as moving genes around plasmids, mutating genes, or mining new genes. The aim is to provide those new to the field with reliable and up-to-date practical guidance while at the same time conveying the scope for creativity. After a brief synopsis of the history of cloning, the fundamentals and prerequisites are explained, covering, for example, software, vectors commonly used in the lab, appropriate choice of restriction endonucleases, the preparation of agarose gels, competent cells, and LB agar plates, and procedures to be followed upon receipt of new plasmids. The remainder of the book is devoted to the clear description of methods and individual steps in cloning. Guidance is provided on the cut and paste method, DNA sequencing, direct sequencing, primer design, PCR-based gene insertion and deletion, epitope tag insertion, the use of RACE technology, BAC recombineering, and much, much more. Sources of error and a variety of techniques that make life considerably easier when cloning are also examined in detail.

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DNA Cloning: A Hands-on Approach

DNA Cloning: A Hands-on Approach

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This book offers step-by-step instruction on DNA cloning, defined as moving genes around plasmids, mutating genes, or mining new genes. The aim is to provide th

DNA Cloning

DNA Cloning

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This book offers step-by-step instruction on DNA cloning, defined as moving genes around plasmids, mutating genes, or mining new genes. The aim is to provide th

DNA Cloning

DNA Cloning

File Size : 88,88 MB
Total View : 5457 Views
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