Enzymes in Nonaqueous Solvents

Released on by Evgeny N. Vulfson
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  • Enzymes in Nonaqueous Solvents Book Detail

  • Author : Evgeny N. Vulfson
  • Release Date : 2008-02-05
  • Publisher : Springer Science & Business Media
  • Genre : Technology & Engineering
  • Pages : 677
  • ISBN 13 : 1592591124
  • File Size : 99,99 MB
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Enzymes in Nonaqueous Solvents by Evgeny N. Vulfson PDF Summary

Book Description: Enzymatic catalysis has gained considerable attention in recent years as an efficient tool in the preparation of natural products, pharmaceuticals, fine chemicals, and food ingredients. The high selectivity and mild reaction con- tions associated with enzymatic transformations have made this approach an attractive alternative in the synthesis of complex bioactive compounds, which are often difficult to obtain by standard chemical routes. However, the maj- ity of organic compounds are not very soluble in water, which was traditi- ally perceived as the only suitable reaction medium for the application of biocatalysts. The realization that most enzymes can function perfectly well under nearly anhydrous conditions and, in addition, display a number of useful properties, e. g. , highly enhanced stability and different selectivity, has d- matically widened the scope of their application to the organic synthesis. Another great attraction of using organic solvents rather than water as a reaction solvent is the ability to perform synthetic transformations with re- tively inexpensive hydrolytic enzymes. It is worth reminding the reader that in vivo, the synthetic and hydrolytic pathways are catalyzed by different enzymes. However, elimination of water from the reaction mixture enables the “reversal” of hydrolytic enzymes and thus avoids the use of the expensive cofactors or activated substrates that are required for their synthetic count- parts.

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Extending the range of enzymatic catalysis by using non-aqueous media has now developed into a powerful approach in biochemistry and biotechnology. One peculiar

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From a biotechnological perspective there are many advantages of employing enzymes in organic as opposed to aqueous media. While there is now little question th