PCR 3

Released on by C. Simon Herrington
preview-18

  • PCR 3 Book Detail

  • Author : C. Simon Herrington
  • Release Date : 1997-10-30
  • Publisher : Oxford University Press
  • Genre : Science
  • Pages : 223
  • ISBN 13 : 0191565598
  • File Size : 3,3 MB
DOWNLOAD BOOK

PCR 3 by C. Simon Herrington PDF Summary

Book Description: PCR in situ hybridization allows the detection of specific nucleic acid sequences and their distribution in the cell. It combines two powerful techniquesin situ hybridization (ISH), which allows cellular localization of DNA and RNA sequences in cells and tissues, and the polymerase chain reaction (PCR), which allows reproducible amplification of rare nucleic acid sequences. The combined technique and its variants greatly enhance the sensitivity of in situ hybridization and add morphological localization to the sensitivity of PCR. Such techniques have enormous potential for research and diagnosis but problems with reproducibility and reliability are often encountered. This book overcomes these problems by describing the key procedures in step-by-step detail and by providing the essential advice needed for success. Topics include: DNA in situ PCR and DNA PCR in situ hybridization (PCR ISH) for the detection of DNA targets in cells; reverse trancriptase in situ PCR (RT-PCR) and RT-PCR ISH for the detection of RNA targets; and PRINS (primed in situ synthesis) for chromosomal analysis in interphase nuclei and metaphase chromosome spreads. There are further chapters on fixation of tissues for PCR, selective ultraviolet radiation fractionation (SURF), application of in situ PCR to human tissues, applications and modifications of PCR-ISH, and automation of in situ amplification. PCR In Situ Hybridization is a unique and timely collection of well-tested protocols for the amplification of DNA and RNA in cells and tissues, drawing on the accumulated knowledge and experience of leading exponents of these techniques. For each topic covered, the authors provide detailed guidance on the key steps in the protocols, numerous hints and tips for success, and advice on trouble-shooting. PCR In Situ Hybridization will be invaluable to molecular biologists, pathologists, geneticists, and all those seeking to perform in situ analyses of nucleic acid molecules.

Disclaimer: www.yourbookbest.com does not own PCR 3 books pdf, neither created or scanned. We just provide the link that is already available on the internet, public domain and in Google Drive. If any way it violates the law or has any issues, then kindly mail us via contact us page to request the removal of the link.

PCR 3

PCR 3

File Size : 35,35 MB
Total View : 3321 Views
DOWNLOAD

PCR in situ hybridization allows the detection of specific nucleic acid sequences and their distribution in the cell. It combines two powerful techniquesin situ

The PCR Revolution

The PCR Revolution

File Size : 50,50 MB
Total View : 5289 Views
DOWNLOAD

Examines the latest innovations and the overall impact of PCR on areas of molecular research.

PCR for Clinical Microbiology

PCR for Clinical Microbiology

File Size : 92,92 MB
Total View : 3397 Views
DOWNLOAD

Not another textbook, but a valuable tool for doctors and microbiologists wanting to know how to set up a PCR diagnostic microbiology laboratory according to cu

PCR

PCR

File Size : 69,69 MB
Total View : 7002 Views
DOWNLOAD

A thoroughly updated version of the successful first edition with a new chapter on Real-Time PCR, more prokaryotic applications, and more detail in the complex

PCR Strategies

PCR Strategies

File Size : 12,12 MB
Total View : 5263 Views
DOWNLOAD

PCR Strategies expands and updates the landmark volume PCR Protocols. It is a companion laboratory manual that provides a completely new set of up-to-date strat