Advanced Photon Counting

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  • Advanced Photon Counting Book Detail

  • Author : Peter Kapusta
  • Release Date : 2015-04-23
  • Publisher : Springer
  • Genre : Science
  • Pages : 371
  • ISBN 13 : 3319156365
  • File Size : 81,81 MB

Advanced Photon Counting by Peter Kapusta PDF Summary

Book Description: This volume focuses on Time-Correlated Single Photon Counting (TCSPC), a powerful tool allowing luminescence lifetime measurements to be made with high temporal resolution, even on single molecules. Combining spectrum and lifetime provides a “fingerprint” for identifying such molecules in the presence of a background. Used together with confocal detection, this permits single-molecule spectroscopy and microscopy in addition to ensemble measurements, opening up an enormous range of hot life science applications such as fluorescence lifetime imaging (FLIM) and measurement of Förster Resonant Energy Transfer (FRET) for the investigation of protein folding and interaction. Several technology-related chapters present both the basics and current state-of-the-art, in particular of TCSPC electronics, photon detectors and lasers. The remaining chapters cover a broad range of applications and methodologies for experiments and data analysis, including the life sciences, defect centers in diamonds, super-resolution microscopy, and optical tomography. The chapters detailing new options arising from the combination of classic TCSPC and fluorescence lifetime with methods based on intensity fluctuation represent a particularly unique highlight.

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Advanced Photon Counting

Advanced Photon Counting

File Size : 76,76 MB
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This volume focuses on Time-Correlated Single Photon Counting (TCSPC), a powerful tool allowing luminescence lifetime measurements to be made with high temporal

Time-correlated single photon counting

Time-correlated single photon counting

File Size : 81,81 MB
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Time-correlated Single Photon Counting has been written in the hope that by relating the authors' experiences with a variety of different single photon counting

Photon-Counting Image Sensors

Photon-Counting Image Sensors

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This book is a printed edition of the Special Issue "Photon-Counting Image Sensors" that was published in Sensors