Genetic Library Construction and Screening

Released on by R.C. Bird
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  • Genetic Library Construction and Screening Book Detail

  • Author : R.C. Bird
  • Release Date : 2012-12-06
  • Publisher : Springer Science & Business Media
  • Genre : Science
  • Pages : 244
  • ISBN 13 : 3642564089
  • File Size : 6,6 MB
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Genetic Library Construction and Screening by R.C. Bird PDF Summary

Book Description: Designed as an introductory text the authors cover all core strategies in the application of modern recombinant DNA technology. The first chapters directly address the applications of polymerase chain reaction to a variety of problems in DNA cloning that are, or have been, extremely challenging using more traditional approaches and technologies. These include cDNA cloning and transcript mapping, mutagenesis as well as the cloning of very long transcripts and protocols using limiting amounts of total RNA. Further chapters describe approaches to subtractive cloning technologies as well as novel specialized expression cloning and library screening strategies. The handbook contains detailed step-by-step protocols and extensive hands-on advice.

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