Recombinant protein expression in microbial systems

Released on by Eduardo A. Ceccarelli
preview-18

  • Recombinant protein expression in microbial systems Book Detail

  • Author : Eduardo A. Ceccarelli
  • Release Date : 2014-10-02
  • Publisher : Frontiers E-books
  • Genre : Biotechnology
  • Pages : 103
  • ISBN 13 : 2889192946
  • File Size : 50,50 MB
DOWNLOAD BOOK

Recombinant protein expression in microbial systems by Eduardo A. Ceccarelli PDF Summary

Book Description: With the advent of recombinant DNA technology, expressing heterologous proteins in microorganisms rapidly became the method of choice for their production at laboratory and industrial scale. Bacteria, yeasts and other hosts can be grown to high biomass levels efficiently and inexpensively. Obtaining high yields of recombinant proteins from this material was only feasible thanks to constant research on microbial genetics and physiology that led to novel strains, plasmids and cultivation strategies. Despite the spectacular expansion of the field, there is still much room for progress. Improving the levels of expression and the solubility of a recombinant protein can be quite challenging. Accumulation of the product in the cell can lead to stress responses which affect cell growth. Buildup of insoluble and biologically inactive aggregates (inclusion bodies) lowers the yield of production. This is particularly true for obtaining membrane proteins or high-molecular weight and multi-domain proteins. Also, obtaining eukaryotic proteins in a prokaryotic background (for example, plant or animal proteins in bacteria) results in a product that lack post-translational modifications, often required for functionality. Changing to a eukaryotic host (yeasts or filamentous fungi) may not be a proper solution since the pattern of sugar modifications is different than in higher eukaryotes. Still, many advances in the last couple of decades have provided to researchers a wide variety of strategies to maximize the production of their recombinant protein of choice. Everything starts with the careful selection of the host. Be it bacteria or yeast, a broad list of strains is available for overcoming codon use bias, incorrect disulfide bond formation, protein toxicity and lack of post-translational modifications. Also, a huge catalog of plasmids allows choosing for different fusion partners for improving solubility, protein secretion, chaperone co-expression, antibiotic resistance and promoter strength. Next, controlling culture conditions like temperature, inducer and media composition can bolster recombinant protein production. With this Research Topic, we aim to provide an encyclopedic account of the existing approaches to the expression of recombinant proteins in microorganisms, highlight recent discoveries and analyze the future prospects of this exciting and ever-growing field.

Disclaimer: www.yourbookbest.com does not own Recombinant protein expression in microbial systems books pdf, neither created or scanned. We just provide the link that is already available on the internet, public domain and in Google Drive. If any way it violates the law or has any issues, then kindly mail us via contact us page to request the removal of the link.

Production of Recombinant Proteins

Production of Recombinant Proteins

File Size : 15,15 MB
Total View : 5706 Views
DOWNLOAD

While the choices of microbial and eukaryotic expression systems for production of recombinant proteins are many, most researchers in academic and industrial se

Expression Systems

Expression Systems

File Size : 96,96 MB
Total View : 6605 Views
DOWNLOAD

1. Expression strategy (Michael Dyson) 2. Protein expression in Escherichia coli (Rosalind Kim) 3. Expression engineering of synthetic antibodies using ribosome

Horizons in Bioprocess Engineering

Horizons in Bioprocess Engineering

File Size : 68,68 MB
Total View : 7363 Views
DOWNLOAD

This book is divided into four parts that outline the use of science and technology for applications pertaining to chemical and bioprocess engineering. The book