CRISPR-Cas system based diagnosis of infectious diseases

Released on by ROOHI BANSAL
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  • CRISPR-Cas system based diagnosis of infectious diseases Book Detail

  • Author : ROOHI BANSAL
  • Release Date : 2021-02-07
  • Publisher : ROOHI BANSAL
  • Genre : Antiques & Collectibles
  • Pages : 214
  • ISBN 13 :
  • File Size : 17,17 MB
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CRISPR-Cas system based diagnosis of infectious diseases by ROOHI BANSAL PDF Summary

Book Description: Since its discovery as a part of the bacterial defense mechanism, the Nobel Prize-winning technology CRISPR-Cas system has revolutionized the fields of genome editing and genetic engineering. Beyond gene-editing ability, scientists have leveraged its potential in the diagnosis of infectious diseases including COVID-19. This book provides a detailed understanding of CRISPR-based rapid and point-of-care diagnostic kits like SHERLOCK, DETECTR, FELUDA, AIOD CRISPR-Cas12a, etc. Although these CRISPR-based tests are performed using isothermal nucleic acid amplification processes like RPA and LAMP, they promise a real-time RT-PCR sensitivity. Furthermore, the tests’ results can be interpreted using paper-based lateral flow strips, potentially reducing laboratory and test costs. In this technique, the colored lines on the strip, similar to pregnancy tests, indicate whether the test is positive or negative. Because of the ease of performing the test and simple interpretation of the test results, CRISPR-based tests can be used at airports, ports, clinics, schools, etc., for better disease diagnosis, monitoring, management, and containment of infectious diseases like COVID-19. Additionally, the book also discusses Monoclonal Antibodies, which have revolutionized the treatment for cancer, arthritis, autoimmune diseases, etc. This book also talks about various strategies to isolate monoclonal antibodies from the COVID-19 recovered people and different ways to engineer these antibodies using hybridoma technology.

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