Molecular Investigations, Cryopreservation and Genetic Transformation Studies in Papaya (Carica Papaya L.) for Cold Hardiness

Released on by Sadanand A. Dhekney
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  • Molecular Investigations, Cryopreservation and Genetic Transformation Studies in Papaya (Carica Papaya L.) for Cold Hardiness Book Detail

  • Author : Sadanand A. Dhekney
  • Release Date : 2004
  • Publisher :
  • Genre :
  • Pages :
  • ISBN 13 :
  • File Size : 78,78 MB
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Molecular Investigations, Cryopreservation and Genetic Transformation Studies in Papaya (Carica Papaya L.) for Cold Hardiness by Sadanand A. Dhekney PDF Summary

Book Description: ABSTRACT: Papaya is one of the major fruit crops of the tropical regions of the world. The cold sensitivity of this crop has limited its cultivation to tropical and warm subtropical regions. The Caricaceae family consists of genera with stress tolerance traits which can be potentially transferred to papaya using a combination of biotechnology and breeding. Carica and Vasconcella genomes were probed for the presence of cold inducible sequences similar to those found in the Arabidopsis genome using the polymerase chain reaction. These studies indicated the presence of possible cold inducible sequences in the Vasconcella genome but which were absent in the Carica genome. A genetic transformation approach was used to transfer CBF transgenes into papaya. The CBF (C repeat Binding Factor) gene family is known to induce the cold acclimation pathway in Arabidopsis. Embryogenic cultures were transformed using the Agrobacterium tumefaciens mediated protocol.

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