Proteomics in Practice

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  • Proteomics in Practice Book Detail

  • Author : Reiner Westermeier
  • Release Date : 2008-09-08
  • Publisher : John Wiley & Sons
  • Genre : Science
  • Pages : 502
  • ISBN 13 : 3527622306
  • File Size : 7,7 MB

Proteomics in Practice by Reiner Westermeier PDF Summary

Book Description: Still the only concise practical guide to laboratory experiments in proteomics, this new edition now also covers DIGE technology and liquid-chromatography, while the troubleshooting section has been considerably extended. Adopting a practical approach, the authors present the relevant techniques and explain the route to successful experimental design and optimal method selection. They cover such electrophoretic techniques as isoelectric focusing, SDS page, 2-D page, and DIGE, as well as liquid-chromatography techniques, such as ion exchange, affinity chromatography and reversed-phase HPLC. Mass-spectrometric techniques include MALDI, ESI, and FT ICR. Generously illustrated, partly in color, the book also features updates of protocols as well as animations illustrating crucial methodological steps on a companion website.

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Introduction to the proteome (K. L. Williams, D. F. Hochstrasser). Two-dimensional electrophoresis: the state of the art and future directions (B. R. Herbert, J