Self-replicating Peptides

Released on by David Hungfai Lee
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  • Self-replicating Peptides Book Detail

  • Author : David Hungfai Lee
  • Release Date : 1998
  • Publisher :
  • Genre : Autopoiesis
  • Pages : 288
  • ISBN 13 : 9780591826104
  • File Size : 28,28 MB
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Self-replicating Peptides by David Hungfai Lee PDF Summary

Book Description: Catalytic and autocatalytic peptides were used to construct non-linear self-reproducing networks. The design of these peptides is based on the coiled coil motif. An alpha-helix, (or a dimeric assembly) acts as a template to preorganize two appropriately activated (self)-complementary peptide fragments, which accelerates their ligation through proximity effects. The catalytic process is sequence- and stereoselective, with observed rate enhancements as high as 4100 fold over background. If the fragments are self-complementary to the template then the ligated product is identical to the catalyst, and the peptide is therefore self-replicating. Since more catalyst is produced, the reaction velocity accelerates, resulting in non-linear growth. Three peptide replicators were studied. Two display parabolic growth, a consequence of product inhibition, while a third is distinguished by near-exponential growth characteristics. The sequence fidelity of the peptide self-replication process was also studied via a direct competition experiment between the original wild-type replicator, two single alanine mutants, and a double alanine mutant, where it was discovered that the mixture self-organized into an autocatalytic network where the single alanine mutants exclusively upregulated the reproduction of the wild-type. Since the wild type peptide subjugates the mutants for its own synthesis, we have dubbed this form of self-organization as an autocratic network. A competition experiment between two self-replicating peptides which share one common fragment was carried out, in order to demonstrate survival-of-the-fittest at the molecular level. Surprisingly however, it was discovered that each peptide, in addition to self-replication, could augment the synthesis of the other, through a hypercyclic network. Because both peptides replicate better in the presence of the other sequence, this hypercyclic network amounts to a symbiotic relationship between the two species. It is hoped that by continuing to explore the properties of novel forms of system-wide organization that we may further our understanding of the organization of biochemical networks in the cell.

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Self-replicating Peptides

Self-replicating Peptides

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Catalytic and autocatalytic peptides were used to construct non-linear self-reproducing networks. The design of these peptides is based on the coiled coil motif

Self-sustained Replication of an RNA Enzyme

Self-sustained Replication of an RNA Enzyme

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RNA enzymes based on the R3C ligase ribozyme motif were previously shown to undergo both self- and cross-catalytic replication, albeit very inefficiently. In an