Single Cell Forensic Genomics

Released on by Haley Hardin
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  • Single Cell Forensic Genomics Book Detail

  • Author : Haley Hardin
  • Release Date : 2020
  • Publisher :
  • Genre :
  • Pages : 72
  • ISBN 13 :
  • File Size : 17,17 MB
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Single Cell Forensic Genomics by Haley Hardin PDF Summary

Book Description: Therefore, enhanced methods for the recovery of sperm cells that allow researchers to bypass the limitations of a typical DNA workflow and obtain an aSTR profile need to be developed. This study aimed to do so by utilizing enhanced, non-standard methods to collect individual sperm cells via direct physical recovery from semen containing samples. Optimized direct lysis and micro-volume aSTR amplification were also used in order to obtain DNA profiles of the sperm donor. The quality of the obtained DNA profiles was evaluated using metrics such as allele recovery, stutter occurrence and percentage, and drop-in allele levels. Using these developed methods, the ability to analyze single sperm cells was demonstrated and the minimum number of sperm cells required in order to obtain probative and reliable DNA profiles was determined.

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